Signosis/UPR/ER Stress Luciferase Reporter Vector Set/LR-3007-2000/1 Ea

来自 : 发布时间：2025-03-10

Description:

The Unfolded Protein Response (UPR) is a conserved and essential stress response that cells activate to combat endoplasmic reticulum (ER) stress, commonly caused by the accumulation of misfolded proteins or failing protein quality control. ER stress is a well-characterized feature of several diseases, such as diabetes, Alzheimer’s, Parkinson’s, Huntingon’s, and prion diseases. Early cellular response to ER stress includes the transcriptional upregulation of chaperone proteins, which is mediated by a large number of transcription factors (TFs).Signosis has developed a set of luciferase reporters (AP1, NFkB, NRF2/ARE, ATF6, XBP1, CHOP, SREBP1 and Negative control) to facilitate UPR/ER stress study.

Principle:

Either the TF promotor (pLuc5U-Oxidative Stress Luciferase Reporter Vector Set) or the TF binding site (All other sets) is upstream of TATA promoter to induce the expression of luciferase gene. It results in the induction of luciferase enzyme activity. Through comparison of luciferase activity in two samples, either TF expression (pLuc5U-Oxidative Stress Luciferase Reporter Vector Set) or the up- or down-regulation of TFs can be quantitatively measured.

Data: