Description:

Anti-nuclearantibodies(ANA)areagroupofantibodiesdirectedagainstvariousnuclearandsomecytoplasmicantigens.Althoughtheseantibodieswerefirstassociatedwithsystemiclupuserythematosus(SLE),thelistofimplicateddiseaseshasexpandedandmanyrheumaticdiseasesarecharacterizedbythepresenceofoneormoreoftheseANAs.Forinstance,anti-SSA/Roandanti-SSB/LaantibodiesareassociatedwithSLEandSjogren"sSyndrome(SS),anti-dsDNAandanti-SmantibodieswithSLE,anti-histoneantibodieswithSLEandDrugInducedLupus,anti-RNPantibodieswithmixedconnectivetissuedisease(MCTD)andSLE,anti-Scl-70antibodieswithscleroderma(progressivesystemicsclerosis(PSSJ),anti-Jo1withpolymyositisanddermatomyositisandanti-centromereantibodieswithCRESTsyndrome.ANAareusuallydetectedbyindirectimmunofluorescence(IFA)onHEp-2.BecauseofcertainlimitationsofIFA,ANAELISAtestismorerobustofferingseveraladvantagesincludingeaseofoperationandnotrequiringskillsneededtoperformandreadIFAreactions.ANAELISAtestisabletoefficientlyscreenlargenumbersofpatientsamplesandreduceshumanerror.AsANAELISAtestcollectivelydetects,inonewell,totalANAsagainstdoublestrandedDNA(dsDNA),Histones,SS-A/Ro,SS-B/La,Sm,Sm/RNP,Scl-70,Jo-1,andcentromericantigens,morespecificantibodytestsarerecommendedtoperforminpatientswithpositiveANA.

Principle:

AutoimmuneELISAkitsmeasureautoimmuneantibodiesintheserum.Itisbasedontheprincipleofasolidphaseenzyme-linkedimmunosorbentassay.Theassayutilizesaspecificantigenforimmobilizationonthemicrotiterwellsandanti-humanIgGantibodyconjugatedtohorserADIshperoxidase(HRP)fordetection.Thetestsampleisallowedtoreactsimultaneouslywiththetwocomponents,resultingin autoimmuneantibodiesbeingsandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Afterincubation,thewellsarewashedtoremoveunbound-labeledantibodies.AHRPsubstrate,TMB,isaddedtoresultinthedevelopmentofabluecolor.ThecolordevelopmentisthenstoppedwiththeadditionofStopSolutionchangingthecolortoyellow.Theconcentrationofautoimmuneantibodies isdirectlyproportionaltothecolorintensityofthetestsample.Absorbanceismeasuredspectrophotometricallyat450nm.

Data:

Signosis 是一家专业的生物分析供应商，致力于基于微孔板分析技术的生命科学研究产品的开发、生产和销售。Signosis 成立于2007年，坐落于世界研发中心--美国硅谷的中心地带。公司专注于细胞因子、转录因子、miRNA及疾病标志物等重要调控因子和标志物的研究，基于自有专利技术，开发完成400多种先进而独特的用于发现、筛选和分析的生命科学研究产品。Signosis 产品销售遍布北美、欧洲、亚洲及中东等主要国家和地区，客户涵盖美国国立卫生研究院、哈佛大学、耶鲁大学等著名研究机构以及众多制药公司