Description:

Mixedconnective-tissuedisease(MCTD)isanautoimmunedisorderwithcoexistenceandoverlapofvariousconnective-tissuediseases(CTDs)suchassystemiclupuserythematosus(SLE);systemicsclerosis(SSc);dermatomyositis(DM);polymyositis(PM);and,occasionally,Sjögrensyndrome.ThepresenceofantibodiestospecificcomponentsofU1-ribonucleoprotein(U1-RNP)complexistheimmunologicalMarkerforthediagnosisofMCTD.ThecomplexiscomposedofU-richedsmallnuclearRNAandasetofproteins,the68kDa(or70kDa)U1-specificproteinplusproteinsAandCandtheSmantigens(B,B`,D1,D2,D3,E,F,andG).Antibodiesagainstthesn-RNPcomplexaredirectedagainstSmaswellasthe70kDaU1-specificproteinsplusproteinsAandC.ItisnowknownthattheavailABIlityofRNPantigenintheabsenceofSmisagoodmarkerforMCTD.

Principle:

AutoimmuneELISAkitsmeasureautoimmuneantibodiesintheserum.Itisbasedontheprincipleofasolidphaseenzyme-linkedimmunosorbentassay.Theassayutilizesaspecificantigenforimmobilizationonthemicrotiterwellsandanti-humanIgGantibodyconjugatedtohorserADIshperoxidase(HRP)fordetection.Thetestsampleisallowedtoreactsimultaneouslywiththetwocomponents,resultingin autoimmuneantibodiesbeingsandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Afterincubation,thewellsarewashedtoremoveunbound-labeledantibodies.AHRPsubstrate,TMB,isaddedtoresultinthedevelopmentofabluecolor.ThecolordevelopmentisthenstoppedwiththeadditionofStopSolutionchangingthecolortoyellow.Theconcentrationofautoimmuneantibodies isdirectlyproportionaltothecolorintensityofthetestsample.Absorbanceismeasuredspectrophotometricallyat450nm.

Data:

Signosis 是一家专业的生物分析供应商，致力于基于微孔板分析技术的生命科学研究产品的开发、生产和销售。Signosis 成立于2007年，坐落于世界研发中心--美国硅谷的中心地带。公司专注于细胞因子、转录因子、miRNA及疾病标志物等重要调控因子和标志物的研究，基于自有专利技术，开发完成400多种先进而独特的用于发现、筛选和分析的生命科学研究产品。Signosis 产品销售遍布北美、欧洲、亚洲及中东等主要国家和地区，客户涵盖美国国立卫生研究院、哈佛大学、耶鲁大学等著名研究机构以及众多制药公司