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当前位置: 首页 > 产品中心 > Target_Expression > Signosis/Mouse Cytokine cDNA Plate Array/AP-1141/1 Ea
商品详细Signosis/Mouse Cytokine cDNA Plate Array/AP-1141/1 Ea
Signosis/Mouse Cytokine cDNA Plate Array/AP-1141/1 Ea
Signosis/Mouse Cytokine cDNA Plate Array/AP-1141/1 Ea
商品编号: AP-1141
品牌: signosisinc
市场价: ¥10480.00
美元价: 6288.00
产地: 美国(厂家直采)
公司:
产品分类: 转录表达
公司分类: Target_Expression
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Description:

CytokinesareessentialmoleculesthatplaycrucialrolesinmanyBIOLOGicalfunctions,includingviralinfection,inflammation,immunity,andhematopoiesis.Cytokinesareproducedbyavarietyofcelltypesinresponsetodifferentstimuli.Inaddition,theexpressionofcytokinegenesappearstoberegulatedbycomplexmechanism.Expressionofonecytokinegenecouldberegulatedbyothercytokines.Dysregulationofcytokinegeneexpressionmaybecausedbychromosomalalterationsorbyinfectionofvirusesthatinduceactivationorinactivationoftheexpressionmachinery.Therefore,expressionprofilingofcytokinegenescouldprovideausefulapproachtouncoverthemechanismunderlyingtheirregulation. 

ApplicableGrid:

ListofApplicableGenes

 123456789101112
A18SrRNAIL-13IL-418SrRNAIL-13IL-418SrRNAIL-13IL-418SrRNAIL-13IL-4
BEotaxinIL-14IL-5EotaxinIL-14IL-5EotaxinIL-14IL-5EotaxinIL-14IL-5
CGAPDH IL-15IL6 GAPDH IL-15IL6 GAPDH IL-15IL6 GAPDH IL-15IL6 
DIFNbIL-16IL-7IFNbIL-16IL-7IFNbIL-16IL-7IFNbIL-16IL-7
EIFNgIL-17TGFaIFNgIL-17TGFaIFNgIL-17TGFaIFNgIL-17TGFa
FIL-10IL1A TGFbIL-10IL1A TGFbIL-10IL1A TGFbIL-10IL1A TGFb
GIL11IL2TNFaIL11IL2TNFaIL11IL2TNFaIL11IL2TNFa
HIL-12IL-3VEGFCIL-12IL-3VEGFCIL-12IL-3VEGFCIL-12IL-3VEGFC

Principle:

Signosis’proprietaryCDNAplatearrayisaplate-basedhybridizationprofilinganalysisformonitoringtheexpressionofdozensofgenesthroughreversetranscriptionofmRNAintocDNA.Likearrayanalyses,totalRNAisfirstreversetranscribedintocDNAinthepresenceofbiotin-dUTPintheassay.Targetedgenesarethenspecificallycapturedontoindividualwellsonaplate,insteadofmembranes,throughapre-coatedgene-specificoligonucleotide.ThecapturedcDNAsarefurtherdetectedwithstreptavidin-HRP.Luminescenceisreportedasrelativelightunits(RLUs)onamicroplateluminometer.Theexpressionlevelofgenesisdirectlyproportionaltotheluminescentintensity.

Data:


MousecytokinecDNAplatearrayanalysis. NIH3T3cellsweretreatedwith(redbars)orwithout(lightblue)10ng/mlTNFfor90min. Cellswerecollectedforprofiling23geneswiththecDNAplatearrayassay.

 
 
品牌介绍
Signosis 是一家专业的生物分析供应商,致力于基于微孔板分析技术的生命科学研究产品的开发、生产和销售。Signosis 成立于2007年,坐落于世界研发中心--美国硅谷的中心地带。公司专注于细胞因子、转录因子、miRNA及疾病标志物等重要调控因子和标志物的研究,基于自有专利技术,开发完成400多种先进而独特的用于发现、筛选和分析的生命科学研究产品。Signosis 产品销售遍布北美、欧洲、亚洲及中东等主要国家和地区,客户涵盖美国国立卫生研究院、哈佛大学、耶鲁大学等著名研究机构以及众多制药公司