Description:
TheWntsignalingpathwayisimportanttobothembryonicdevelopmentandtumOrigenesis.ß-Catenin,thecentralcomponentofthepathway,functionsasaco-factoroftheTCF/LEFfamilyoftranscriptionfactors. Together,theyactivatetranscriptionofWnttargetgenesbybindingtothepromotersofdownstreamtargetgenesinvolvedincellproliferation,survival,andmigration.Signosis’Wnt/ß-CateninsignalingpathwayCDNAplatearraycansimultaneouslymeasuretheexpressionof20+genesthatparticipateinthepathwayandcancomparethedifferencesintheexpressionofthesegenesindifferentsamples.ApplicableGrid:
ListofApplicableGenes
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | Axin-2 | Id2 | NOS2 | Axin-2 | Id2 | NOS2 | Axin-2 | Id2 | NOS2 | Axin-2 | Id2 | NOS2 |
| B | beta-actin | LEF1 | POU5F1 | beta-actin | LEF1 | POU5F1 | beta-actin | LEF1 | POU5F1 | beta-actin | LEF1 | POU5F1 |
| C | beta-catenin | Met | PPARd | beta-catenin | Met | PPARd | beta-catenin | Met | PPARd | beta-catenin | Met | PPARd |
| D | c-JUN | MMP1 | Sox9 | c-JUN | MMP1 | Sox9 | c-JUN | MMP1 | Sox9 | c-JUN | MMP1 | Sox9 |
| E | c-myc | MMP-7 | Survivin | c-myc | MMP-7 | Survivin | c-myc | MMP-7 | Survivin | c-myc | MMP-7 | Survivin |
| F | CyclinD | MMP9 | Tcf-1 | CyclinD | MMP9 | Tcf-1 | CyclinD | MMP9 | Tcf-1 | CyclinD | MMP9 | Tcf-1 |
| G | FasL1 | MMP26 | uPAR | FosL1 | MMP26 | uPAR | FosL1 | MMP26 | uPAR | FosL1 | MMP26 | uPAR |
| H | Blank | nanog | VEGF | Blank | nanog | VEGF | Blank | nanog | VEGF | Blank | nanog | VEGF |
Principle:
Signosis’proprietarycDNAplatearrayisaplate-basedhybridizationprofilinganalysisformonitoringtheexpressionofdozensofgenesthroughreversetranscriptionofmRNAintocDNA.Likearrayanalyses,totalRNAisfirstreversetranscribedintocDNAinthepresenceofbiotin-dUTPintheassay.Targetedgenesarethenspecificallycapturedontoindividualwellsonaplate,insteadofmembranes,throughapre-coatedgene-specificoligonucleotide.ThecapturedcDNAsarefurtherdetectedwithstreptavidin-HRP.Luminescenceisreportedasrelativelightunits(RLUs)onamicroplateluminometer.Theexpressionlevelofgenesisdirectlyproportionaltotheluminescentintensity. |
Data:
HumanWntcDNAplatearrayanalysis. HeLacellsweretreatedwithorwithout2uMLPAfor8hours. Cellswerecollectedforprofiling23geneswiththecDNAplatearrayassay.
