Description:
SignalTransducerandActivatorofTranscription3(Stat3)isatranscriptionfactorthatmediatesthenuclearactionofmanydifferentcytokinesandgrowthfactors.Itplaysrolesincellgrowthandapoptosis.Stat3alsoparticipatesincellulartransformationandtumOrigenesis.TheaberrantactivationofStat3intumorcellshasbeenconsideredasadrugtarget.Stat3isactivatedbytyrosinephosphorylationinresponsetoawiderangeofcytokines,growthfactors,andhormones.Tyrosinephosphorylationtriggersdimerizationandnucleartranslocationoftheprotein.Stat3mediatestheexpressionofitstargetgenesthatareinvolvedinadiversearrayofBIOLOGicalprocessessuchasoncogenesis,cellgrowth,anddifferentiation.ApplicableGrid:
ListofApplicableGenes
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | A2M | c-myc | Jun | A2M | c-myc | Jun | A2M | c-myc | Jun | A2M | c-myc | Jun |
| B | CCND1 | CRP | JunB | CCND1 | CRP | JunB | CCND1 | CRP | JunB | CCND1 | CRP | JunB |
| C | b-actin | cyclinE | Mcl-1 | b-actin | cyclinE | Mcl-1 | b-actin | cyclinE | Mcl-1 | b-actin | cyclinE | Mcl-1 |
| D | Bad | DNMT1 | OSM | Bad | DNMT1 | OSM | Bad | DNMT1 | OSM | Bad | DNMT1 | OSM |
| E | Bax | GAPDH | p21 | Bax | GAPDH | p21 | Bax | GAPDH | p21 | Bax | GAPDH | p21 |
| F | Bcl-2 | gp130 | p27 | Bcl-2 | gp130 | p27 | Bcl-2 | gp130 | p27 | Bcl-2 | gp130 | p27 |
| G | Bcl-xL | GSK-3beta | Survivine | Bcl-xL | GSK-3beta | Survivine | Bcl-xL | GSK-3beta | Survivine | Bcl-xL | GSK-3beta | Survivine |
| H | c-fos | IL-10 | Blank | c-fos | IL-10 | Blank | c-fos | IL-10 | Blank | c-fos | IL-10 | Blank |
Principle:
Signosis’proprietaryCDNAplatearrayisaplate-basedhybridizationprofilinganalysisformonitoringtheexpressionofdozensofgenesthroughreversetranscriptionofmRNAintocDNA.Likearrayanalyses,totalRNAisfirstreversetranscribedintocDNAinthepresenceofbiotin-dUTPintheassay.Targetedgenesarethenspecificallycapturedontoindividualwellsonaplate,insteadofmembranes,throughapre-coatedgene-specificoligonucleotide.ThecapturedcDNAsarefurtherdetectedwithstreptavidin-HRP.Luminescenceisreportedasrelativelightunits(RLUs)onamicroplateluminometer.Theexpressionlevelofgenesisdirectlyproportionaltotheluminescentintensity. |
Data:
HumanStat3-regulatedcDNAplatearrayanalysis. HeLacellsweretreatedwithandwithout100ng/mlOncostatinMfor1hours,RNAsprepared,cDNAsynthesizedwithbiotinlabelandsubjectedtocDNAplatearrayhybridizationanddetection.
