Description:

RANTES is a chemotactic protein for T cells, eosinophils, and basophils. RANTES is produced by circulating T-cells. The synthesis of RANTES is induced by TNF-alpha and IL1-alpha. It plays an active role in recruiting leukocytes into inflammatory sites. With the help of particular cytokines that are released by T cells, Rantes also induces the proliferation and activation of certain natural-killer (NK) cells to form CC-Chemokine-activated killer cells. It is also an HIV-suppressive factor released from CD8+ T cells. RANTES increases the adherence of monocytes to endothelial cells.

Principle:

The ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes a anti-rat cytokine antibody for immobilization on the microtiter wells and anti-rat cytokine antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the molecules of the cytokine being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of the cytokine is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

 

 

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