Description:

TumourNecrosisFactoralpha(TNFa),isaninflammatorycytokineproducedbymacrophages/monocytesduringacuteinflammationandisresponsIBLeforadiverserangeofsignalingeventswithincells,leADIngtonecrosisorapoptosis.Theproteinisalsoimportantforangiogenesisthatiscriticaltothegrowth,progression,andmetastasisofsolidtumors. FurThermore,TNFaisassociatedwithobesity. Itischronicallyelevatedinadiposetissuesofobeserodentsandhumansandmayrepresentanimportantlinkbetweenobesityandinsulinresistance. Inbothobesemiceandhumans,TNFaisoverexpressedinadiposetissue. TNFainhibitsinsulinsignaling,atleastinpartbyblockinginsulinreceptortyrosinekinaseactivityandinducingserinephosphorylationofinsulinreceptorsubstrate-1(IRS-1).However,itisunclearwhatthephysiologicalstimulatorofTNF-aproductionbyadipocyteduringobesityisandhowIRS-1inhibitsthetyrosinekinaseactivityoftheinsulinreceptorafterTNF-atreatmentofthecells.Abetterunderstandingoftheconnection(s)betweentheTNF-aandtheinsulinsignalingpathwayscouldbeimportanttofindacureforthestateofinsulinresistanceobservedduringobesity.

Principle:

Mouse CytokineELISAisbasedontheprincipleofasolidphaseenzyme-linkedimmunosorbentassay.Theassayutilizesrabbitanti-mouseantibodiesforimmobilizationonthemicrotiterwellsandrabbitanti-mouseantibodiesalongwithstreptavidinconjugatedtohorseradishperoxidase(HRP)fordetection.Thetestsampleisallowedtoreactsimultaneouslywiththetwoantibodies,resultinginthemoleculesbeingsandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Afterincubation,thewellsarewashedtoremoveunbound-labeledantibodies.AHRPsubstrate,TMB,isaddedtoresultinthedevelopmentofabluecolor.ThecolordevelopmentisthenstoppedwiththeadditionofStopSolutionchangingthecolortoyellow.The  concentrationofVEGFisdirectlyproportionaltothecolorintensityofthetestsample.Absorbanceismeasuredspectrophotometricallyat450nm. 
 

 

 

 

Data:

 

 

 

Signosis 是一家专业的生物分析供应商，致力于基于微孔板分析技术的生命科学研究产品的开发、生产和销售。Signosis 成立于2007年，坐落于世界研发中心--美国硅谷的中心地带。公司专注于细胞因子、转录因子、miRNA及疾病标志物等重要调控因子和标志物的研究，基于自有专利技术，开发完成400多种先进而独特的用于发现、筛选和分析的生命科学研究产品。Signosis 产品销售遍布北美、欧洲、亚洲及中东等主要国家和地区，客户涵盖美国国立卫生研究院、哈佛大学、耶鲁大学等著名研究机构以及众多制药公司