Description:

Interferons(IFNs)arepotentextracellularproteinmediatorsofhostdefenceandhomoeostasis.Theyarecytokinesproducedbythecellsoftheimmunesysteminresponsetochallengesbyforeignagentssuchasviruses,parasitesandtumorcells.Itisproducedbyawidevarietyofcellsinresponsetothepresenceofdouble-strandedRNA,akeyindicatorofviralinfection.IFNsaredividedintotwomajorsubgroups.TypeIIFNsallbindtoatypeIIFNreceptor,suchasIFN-aandIFN-ß.IFN-yisthesoletypeIIIFN,whichbindstoadistincttypeIIreceptor.AlmostallcelltypesproducetypeIIFNs,whilethetypeIIIFN-yisproducedinTcellsandnaturalkiller(NK)cellsuponimmunologicalstimulation.IFN-ycoordinatesadiversearrayofcellularprogramsthroughtranscriptionalregulationofimmunologicallyrelevantgenes.CellulareffectsofIFN-yincludesup-regulationofpathogenrecognition,antigenprocessingandpresentation,theantiviralstate,inhibitionofcellularproliferationandeffectsonapoptosis,activationofmicrobicidaleffectorfunctions,immunomodulation,andleukocytetrafficking.

Principle:

TheELISAisbasedontheprincipleofasolidphaseenzyme-linkedimmunosorbentassay.Theassayutilizesamouseanti-humancytokineantibodyforimmobilizationonthemicrotiterwellsandgoatanti-humancytokineantibodiesalongwithstreptavidinconjugatedtohorserADIshperoxidase(HRP)fordetection.Thetestsampleisallowedtoreactsimultaneouslywiththetwoantibodies,resultinginthemoleculesofthecytokinebeingsandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Afterincubation,thewellsarewashedtoremoveunbound-labeledantibodies.AHRPsubstrate,TMB,isaddedtoresultinthedevelopmentofabluecolor.ThecolordevelopmentisthenstoppedwiththeadditionofStopSolutionchangingthecolortoyellow.Theconcentrationofthecytokineisdirectlyproportionaltothecolorintensityofthetestsample.Absorbanceismeasuredspectrophotometricallyat450nm.

Data:

 

 

 

Signosis 是一家专业的生物分析供应商，致力于基于微孔板分析技术的生命科学研究产品的开发、生产和销售。Signosis 成立于2007年，坐落于世界研发中心--美国硅谷的中心地带。公司专注于细胞因子、转录因子、miRNA及疾病标志物等重要调控因子和标志物的研究，基于自有专利技术，开发完成400多种先进而独特的用于发现、筛选和分析的生命科学研究产品。Signosis 产品销售遍布北美、欧洲、亚洲及中东等主要国家和地区，客户涵盖美国国立卫生研究院、哈佛大学、耶鲁大学等著名研究机构以及众多制药公司