Description:
Growth factors are a family of proteins capable of stimulating cellular growth, proliferation, and cellular differentiation. They typically act as signaling molecules by binding to receptors on the cell surface. Many growth factors are quite versatile, stimulating cellular division in numerous different cell types; while others are specific to a particular cell-type. Multiple growth factors often work together to play their roles. For example, VEGF, FGF, and EGF stimulate angiogenesis. Therefore, profiling the expression pattern of growth factors provides a valuable insight to the biological mechanisms underlying cellular functions. Signosis’ Growth Factor ELISA Strip allows quantitatively profiling and measuring 8 growth factor cytokines.Principle:
Each well of the strip is coated with a specific capture antibody to detect its corresponding cytokine in the sample. Therefore, 8 different proteins can be measures simultaneously. The test sample reacts simultaneously with two antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. The HRP substrate, TMB, is then added and causes a blue color change. The reaction is then terminated with Stop Solution, resulting in a yellow color. The concentrations of oxidative stress cytokines are directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. The expression levels of these cytokines can be quantitatively compared between samples.
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