Description:

Angiogenesisprovidesthecruciallinkbetweentheavascularandvascularstatesandisakeyeventforsustainedtumorgrowthandcancerprogression.AngiogenesisasaBIOLOGicalswitchprocessisgovernedbynumerouspro-andanti-angiogenicfactors,includingvascularendothelialgrowthfactor(VEGF),basicfibroblastgrowthfactor(FGFb),epidermalgrowthfactor(EGF),andtransforminggrowthfactor-beta(TGF-b).Themechanismofactionofeachofthesefactorsisdifferent,asaretheiroriginandthestimulifortheirproduction.Theangiogenicswitchreferstothebalancebetweenpro-andanti-angiogenicfactors.Therefore,profilingofthesefactorsiscriticaltounderstandingangiogenesis.Signosis’AngiogenesisELISAStripIProfilingAssayallowssimultaneouslyprofiling8angiogenesiscytokines. Eachwellofthestripiscoatedwithaprimaryantibodyagainstaspecificangiogenesisproteinandtotal8wellsofastriptarget8differentproteins.Thedifferenceoftheseproteinsbetweentwosamplescanbedeterminedthroughdatacomparison.

Principle:

Eachwellofthestripiscoatedwithaspecificcaptureantibodytodetectitscorrespondingcytokineinthesample.Therefore,8differentproteinscanbemeasuressimultaneously.Thetestsamplereactssimultaneouslywithtwoantibodies,resultinginthecytokinesbeingsandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Afterincubation,thewellsarewashedtoremoveunbound-labeledantibodies.TheHRPsubstrate,TMB,isthenaddedandcausesabluecolorchange.ThereactionisthenterminatedwithStopSolution,resultinginayellowcolor.Theconcentrationsofoxidativestresscytokinesaredirectlyproportionaltothecolorintensityofthetestsample.Absorbanceismeasuredspectrophotometricallyat450nm.Theexpressionlevelsofthesecytokinescanbequantitativelycomparedbetweensamples.

 

 

 

Data:

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

HumanAngiogenesisELISAstripIforprofiling8cytokines.HeLacellsweretreatedwith(redbars)orwithout(lightblue)10ng/mlTNF.Conditionedmediawerecollected(top)andcelllysateswereprepared(bottom)forELISAstripprofiling.

 

 

Signosis 是一家专业的生物分析供应商，致力于基于微孔板分析技术的生命科学研究产品的开发、生产和销售。Signosis 成立于2007年，坐落于世界研发中心--美国硅谷的中心地带。公司专注于细胞因子、转录因子、miRNA及疾病标志物等重要调控因子和标志物的研究，基于自有专利技术，开发完成400多种先进而独特的用于发现、筛选和分析的生命科学研究产品。Signosis 产品销售遍布北美、欧洲、亚洲及中东等主要国家和地区，客户涵盖美国国立卫生研究院、哈佛大学、耶鲁大学等著名研究机构以及众多制药公司