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Signosis/Human Angiogenesis ELISA Strip I (Colorimetric)/EA-1011/1 Ea
Description:
Angiogenesisprovidesthecruciallinkbetweentheavascularandvascularstatesandisakeyeventforsustainedtumorgrowthandcancerprogression.AngiogenesisasaBIOLOGicalswitchprocessisgovernedbynumerouspro-andanti-angiogenicfactors,includingvascularendothelialgrowthfactor(VEGF),basicfibroblastgrowthfactor(FGFb),epidermalgrowthfactor(EGF),andtransforminggrowthfactor-beta(TGF-b).Themechanismofactionofeachofthesefactorsisdifferent,asaretheiroriginandthestimulifortheirproduction.Theangiogenicswitchreferstothebalancebetweenpro-andanti-angiogenicfactors.Therefore,profilingofthesefactorsiscriticaltounderstandingangiogenesis.Signosis’AngiogenesisELISAStripIProfilingAssayallowssimultaneouslyprofiling8angiogenesiscytokines. Eachwellofthestripiscoatedwithaprimaryantibodyagainstaspecificangiogenesisproteinandtotal8wellsofastriptarget8differentproteins.Thedifferenceoftheseproteinsbetweentwosamplescanbedeterminedthroughdatacomparison.Principle:
Eachwellofthestripiscoatedwithaspecificcaptureantibodytodetectitscorrespondingcytokineinthesample.Therefore,8differentproteinscanbemeasuressimultaneously.Thetestsamplereactssimultaneouslywithtwoantibodies,resultinginthecytokinesbeingsandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Afterincubation,thewellsarewashedtoremoveunbound-labeledantibodies.TheHRPsubstrate,TMB,isthenaddedandcausesabluecolorchange.ThereactionisthenterminatedwithStopSolution,resultinginayellowcolor.Theconcentrationsofoxidativestresscytokinesaredirectlyproportionaltothecolorintensityofthetestsample.Absorbanceismeasuredspectrophotometricallyat450nm.Theexpressionlevelsofthesecytokinescanbequantitativelycomparedbetweensamples.
Data:
HumanAngiogenesisELISAstripIforprofiling8cytokines.HeLacellsweretreatedwith(redbars)orwithout(lightblue)10ng/mlTNF.Conditionedmediawerecollected(top)andcelllysateswereprepared(bottom)forELISAstripprofiling.