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Signosis/Human Interleukin ELISA Strip (Chemiluminescence)/EA-1681/1 Ea
Description:
Interleukinsareafamilyofchemicalmessengersthatcanevokeawidespectrumofresponsesinthebody,includingimmuneandinflammatoryresponses. UnderstandingtheconditionsthataltertheirexpressionscanbecriticalforunravelingtheunderlyingmechanismsofdiseasessuchasRheumatoidArthritis,Atherosclerosis,andavarietyofcancers. Signosis’sInterleukinELISAStripProfilingAssaycansimultaneouslyanalyze8interleukins. Eachwellofthestripiscoatedwithaspecificcaptureantibodytodetectcorrespondingcytokineinthesample.Theexpressionlevelsofthesecytokinescanbequantitativelycomparedbetweensamples.Principle:
Eachwellofthestripiscoatedwithaspecificcaptureantibodytodetectitscorrespondingcytokineinthesample.Therefore,8differentproteinscanbemeasuressimultaneously.Thetestsamplereactssimultaneouslywithtwoantibodies,resultinginthecytokinesbeingsandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Afterincubation,thewellsarewashedtoremoveunbound-labeledantibodies.TheHRPsubstrate,TMB,isthenaddedandcausesabluecolorchange.ThereactionisthenterminatedwithStopSolution,resultinginayellowcolor.Theconcentrationsofoxidativestresscytokinesaredirectlyproportionaltothecolorintensityofthetestsample.Absorbanceismeasuredspectrophotometricallyat450nm.Theexpressionlevelsofthesecytokinescanbequantitativelycomparedbetweensamples.
Data:
ThreedifferentpatientsampleswereanalyzedusingtheHumanInterleukinELISAStripforProfiling8Cytokines.
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