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Signosis/Human Oxidative Stress ELISA Strip (Colorimetric)/EA-1301/1 Ea
Description:
Oxidativestresscandirectlydamagecellstructuresandmayleadtocancer,inflammatorydiseases,andaging.Interestingly,oxidativestresscanalsoaltertheexpressionlevelsofavarietyofcytokines.Understandingtheconditionsthataltertheexpressionofthesecellularmessengersisimportantforunravelingthemechanismsofthesediseasesanddevelopingtherapeutics. Signosis’sOxidativeStressELISAStripProfilingAssaycansimultaneouslyanalyze8cytokines. Eachwellofthestripiscoatedwithaspecificcaptureantibodytodetectcorrespondingcytokineinthesample.Theexpressionlevelsofthesecytokinescanbequantitativelycomparedbetweensamples.Principle:
Eachwellofthestripiscoatedwithaspecificcaptureantibodytodetectitscorrespondingcytokineinthesample.Therefore,8differentproteinscanbemeasuressimultaneously.Thetestsamplereactssimultaneouslywithtwoantibodies,resultinginthecytokinesbeingsandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Afterincubation,thewellsarewashedtoremoveunbound-labeledantibodies.TheHRPsubstrate,TMB,isthenaddedandcausesabluecolorchange.ThereactionisthenterminatedwithStopSolution,resultinginayellowcolor.Theconcentrationsofoxidativestresscytokinesaredirectlyproportionaltothecolorintensityofthetestsample.Absorbanceismeasuredspectrophotometricallyat450nm.Theexpressionlevelsofthesecytokinescanbequantitativelycomparedbetweensamples.
Data:
ThreedifferentpatientsampleswereanalyzedusingtheHumanOxidativeStressELISAStripforProfiling8Cytokines.