Description:

CytokinesaresignalingmoleculesthathavecriticalrolesinmanyBIOLOGicalprocessessuchascellulargrowth,differentiation,geneexpression,migration,immunity,andinflammation.Cytokinesthataresecretedfromcellsbindtocell-surfacereceptors,initiatetheactivationofsignaltransductionpathwaysandmediatecelltocellcommunication.Themalfunctionofcytokinesleadstomanydiseases,includingarthritis,acuteandchronicliverdisease,inflammatoryboweldisease,cardiac-relateddiseases,andcancers.Cytokinesarecommonlyworkingtogetherinabiologicalordiseaseprocess.Therefore,thecomprehensiveanalysisoftheexpressionofmultiplecytokinesallowseffectiverevealingoftheunderneathmechanismofcytokineactionandthealterationleADIngtodiseases.TheHumanCytokineELISAPlateArrayisachemiluminescentdetectionthatallowsyoutomonitortheabundanceof32humancytokinessimultaneously.Thisfastandsensitiveassaycanbeusedforquantitativecomparisonofthesecytokinesamongdifferentsamples.

ApplicableGrid:

ListofApplicableCytokines

1

2

3

4

5

6

7

8

9

10

11

12

A

TNFa

VEGF

PDGF-BB

IL-10

TNFa

VEGF

PDGF-BB

IL-10

TNFa

VEGF

PDGF-BB

IL-10

B

IFNy

EGF

PIGF-1

FGFb

IFNy

EGF

PLGF-1

FGFb

IFNy

EGF

PLGF-1

FGFb

C

G-CSF

IL-6

b-NGF

Leptin

G-CSF

IL-6

b-NGF

Leptin

G-CSF

IL-6

b-NGF

Leptin

D

GM-CSF

Resistin

SCF

IGF-1

GM-CSF

Resistin

SCF

IGF-1

GM-CSF

Resistin

SCF

IGF-1

E

IL-1a

PAI-1

MCP-1

TGF-b

IL-1a

PAI-1

MCP-1

TGF-b

IL-1a

PAI-1

MCP-1

TGF-b

F

IL-8

IL-12

MIP-1a

Adipo

IL-8

IL-12

MIP-1a

Adipo

IL-8

IL-12

MIP-1a

Adipo

G

IP-10

IL-13

IL-2

IL-17a

IP-10

IL-13

IL-2

IL-17a

IP-10

IL-13

IL-2

IL-17a

H

Rantes

Eotaxin-3

IL-4

IL-1b

Rantes

Eotaxin-3

IL-4

IL-1b

Rantes

Eotaxin-3

IL-4

IL-1b

Principle:

The96-wellwhiteplateisdividedinto3or4sections,andeachsectionhas4or3columnsforonesample.Ineachsection,32(HumanCytokineELISAPlateArrays)or24(MouseCytokineELISAPlateArray)specificcytokinecaptureantibodiesarecoatedon32or24wellsrespectively.Thesample,cellculturesupernatants,celllysates,tissuehomogenates,serum,orplasmasamplesamongothers areincubatedwiththecytokineELISAplate,and thecapturedcytokineproteinsaresubsequentlydetectedwithacocktailofbiotinylateddetectionantibodies.Thetestsampleisallowedtoreactwith apairof antibodies,resultinginthecytokinesbeingsandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Afterincubation,thewellsarewashedtoremoveunbound-labeledantibodies.TheplateisfurtherdetectedwithHRPluminescentsubstrateorHRPsubstrateTMB.Thelevelofexpressionforeachspecificcytokineisdirectlyproportionaltotheluminescentorcolor intensity.

 

Data:

AnalysisofCytokineProteinExpressioninTNFa-TreatedandUntreatedHeLawithHumanCytokineELISAPlateArrayHeLacellswerestarvedfor24hourswithserum-freemedium,subsequentlytreatedthecellswithandwithout20ng/ulTNFfor16hours.Theserum-freeconditionedmediawereincubatedontheplatefor1hour.AfterincubatingwithdetectionantibodymixandHRP,theplatewasdetectedwithchemilumincentsubstratebyaplatereader.

Signosis 是一家专业的生物分析供应商，致力于基于微孔板分析技术的生命科学研究产品的开发、生产和销售。Signosis 成立于2007年，坐落于世界研发中心--美国硅谷的中心地带。公司专注于细胞因子、转录因子、miRNA及疾病标志物等重要调控因子和标志物的研究，基于自有专利技术，开发完成400多种先进而独特的用于发现、筛选和分析的生命科学研究产品。Signosis 产品销售遍布北美、欧洲、亚洲及中东等主要国家和地区，客户涵盖美国国立卫生研究院、哈佛大学、耶鲁大学等著名研究机构以及众多制药公司