Description:

TFEB is a member of the basic Helix-Loop-Helix-Zipper family of transcription factors that specifically recognizes and binds E-box sequences (5'-CANNTG-3') on DNA. TFEB is a master gene for lysosomal biogenesis, and therefore plays a central role in regulating expression of lysosomal genes. TFEB also acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Under aberrant lysosomal storage conditions such as in lysosomal storage diseases, TFEB is translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes. Signosis has developed the TFEB ELISA kits for sensitive and specific analysis of the activities of TFEB in a high throughput manner. The kit can be used for human, mouse and rat samples.

Applicable Grid:

ELISA Kits Available:

TFEB ELISA Kit (Colorimetric) TE-0029

TFEB ELISA Kit (Chemiluminescence) TE-0030

Principle:

TF ELISA kit is high sensitive and specific assay with a simple and optimized procedure. The 96-well (8X12 strip) clear plate is pre-immobilized with the TF consensus sequencing oligo. The activated TF in nuclear extract or the whole cell lysate is added in the well and binds to the oligo. The activated TF is detected with a specific antibody against the subunit and a HRP conjugated secondary antibody. The assay utilizes colorimetric detection method, which can be easily measured by spectrophotometry.

 

Signosis 是一家专业的生物分析供应商，致力于基于微孔板分析技术的生命科学研究产品的开发、生产和销售。Signosis 成立于2007年，坐落于世界研发中心--美国硅谷的中心地带。公司专注于细胞因子、转录因子、miRNA及疾病标志物等重要调控因子和标志物的研究，基于自有专利技术，开发完成400多种先进而独特的用于发现、筛选和分析的生命科学研究产品。Signosis 产品销售遍布北美、欧洲、亚洲及中东等主要国家和地区，客户涵盖美国国立卫生研究院、哈佛大学、耶鲁大学等著名研究机构以及众多制药公司