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Signosis/Stem Cell TF Activation Profiling Plate Array I with Nuclear Extraction Kit/FA-1003-NE/1 Ea.带核提取试剂盒的Signosis/Stem Cell TF活化模板阵列Ⅰ
Signosis/Stem Cell TF Activation Profiling Plate Array I with Nuclear Extraction Kit/FA-1003-NE/1 Ea.带核提取试剂盒的Signosis/Stem Cell TF活化模板阵列Ⅰ
商品编号：
FA-1003-NE
品牌：
signosisinc
市场价：
¥15300.00
美元价：
9180.00
产品分类：
基因突变
公司分类：
Genetic_mutations
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:
StemcellsareimportantcellscharacterizedbytheirABIlitytoself-renewordifferentiateintomanycelltypes,whichisgovernedbyintracellularsignalingpathwayandtranscriptionregulation.Hence,transcriptionregulationplaysadeterminantroleinconferringcellularidentityandfunction.Thestep-wisematurationofstemcellsintoterminallydifferentiatedcelltypesrequiresthetimelyactivationofacascadeoftranscriptionprogramsgovernedbylineage-specifyingtranscriptionfactors.Multipletranscriptionfactorsandtargetgeneshavebeenwidelyreportedtoassociatewithstemcellself-renewalandpluripotency,includingEGR1,OCT4,FOXD3,FOXO,Nanog,SOX2,SOX18,ETS,GLI,KLF4,MEF2,Myc,RUNX1,Pax6,TCF/LEFandGATA.AnalyzingtheactivitiesoftheseTFscanprovidevaluablecluesinthestudyandmanipulationofstemcells.SignosishasdevelopedStemCellTranscriptionFactorActivationPlateArraytoanalyzetheactivitiesof16stemcell-specificTFssimultaneouslyinmammaliansamples.Theassaycanbeusedwiththewholecelllysisfrom1000-10000cells.ApplicableGrid:
ListofApplicableTFs:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | EGR1 | Ets | EGR1 | Ets | EGR1 | Ets | EGR1 | Ets | EGR1 | Ets | EGR1 | Ets |
| B | FoxD3 | Gli | FoxD3 | Gli | FoxD3 | Gli | FoxD3 | Gli | FoxD3 | Gli | FoxD3 | Gli |
| C | FoxO1 | KLF4 | FoxO1 | KLF4 | FoxO1 | KLF4 | FoxO1 | KLF4 | FoxO1 | KLF4 | FoxO1 | KLF4 |
| D | GATA | MEF2 | GATA | MEF2 | GATA | MEF2 | GATA | MEF2 | GATA | MEF2 | GATA | MEF2 |
| E | Nanog | Myc | Nanog | Myc | Nanog | Myc | Nanog | Myc | Nanog | Myc | Nanog | Myc |
| F | OCT4 | RUNX1 | OCT4 | RUNX1 | OCT4 | RUNX1 | OCT4 | RUNX1 | OCT4 | RUNX1 | OCT4 | RUNX1 |
| G | SOX2 | Pax6 | SOX2 | Pax6 | SOX2 | Pax6 | SOX2 | Pax6 | SOX2 | Pax6 | SOX2 | Pax6 |
| H | SOX18 | TCF/LEF | SOX18 | TCF/LEF | SOX18 | TCF/LEF | SOX18 | TCF/LEF | OX18 | TCF/LEF | SOX18 | TCF/LEF |
Principle:
Signosis’TFActivationProfilingArraysareusedformonitoringtheactivitiesofmultipleTFssimultaneously.Inthetechnology,aseriesofbiotin-labeledprobesaremadebasedontheconsensussequencesofTFDNA-bindingsites.Whentheprobemixturesincubatewithnuclearextracts,individualprobeswillfindtheircorrespondingTFsandformTF/probecomplexes,whichcanbeeasilyseparatedfromfreeprobesthroughasimplespincolumnpurification.Theboundprobesarethendetachedfromthecomplexesandanalyzedthroughhybridizationwithaplate,whicheachwellisspecificallypre-coatedwithcomplementarysequencesoftheprobes.
品牌介绍
Signosis 是一家专业的生物分析供应商，致力于基于微孔板分析技术的生命科学研究产品的开发、生产和销售。Signosis 成立于2007年，坐落于世界研发中心--美国硅谷的中心地带。公司专注于细胞因子、转录因子、miRNA及疾病标志物等重要调控因子和标志物的研究，基于自有专利技术，开发完成400多种先进而独特的用于发现、筛选和分析的生命科学研究产品。Signosis 产品销售遍布北美、欧洲、亚洲及中东等主要国家和地区，客户涵盖美国国立卫生研究院、哈佛大学、耶鲁大学等著名研究机构以及众多制药公司
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