Description:
SFRLuciferaseReporterHEK293CellLineisderivedfromhumanembryonicKidney,andstablyexpressfireflyluciferasereportergeneunderthecontrolofSFRresponseelement.ThiscelllineisanidealcellularmodelformonitoringtheactivationofSFRresponsePathwaytriggeredbystimulitreatment,enforcedgeneexpressionandgeneknockdown.Principle:
Serumresponsefactor(SRF)isamemberofthehighlyconservedMADSboxfamilyoftranscriptionfactorswhichregulatestheexpressionofimmediateearlygenes.SRFbindstoitsspecificpromotersequence(CArGbox).SRFencodesaubiquitousnuclearproteinthatstimulatesbothcellproliferationanddifferentiation.SRFbindstotheserumresponseelement(SRE)inthepromoterregionoftargetgenes,regulatingtheactivityofmanyimmediate-earlygenes,forexamplec-fosandactin,therebyparticipatesincellcycleregulation,apoptosis,cellgrowth,andcelldifferentiation,andcytoskeletalregulation. Thisgeneisthedownstreamtargetofmanypathways;forexample,SRFco-activatorsareactivatedbytheMAPKpathwaythroughtheternarycomplexfactors(TCFs)inresponsetomitogenicandstressstimuli.Inaddition,SRFspecificco-activatorstheMAL/MRTFproteinsarereleasedfromarepressivecomplexwithG-actinuponinducedchangesinactindynamics.Thefullpictureofthebroadanddiverseuseofasinglefactor,SRF,awaitedthepoweroftoday’sgenomicandcellularapproaches.
SignosisoffersastablecelllinethatallowsyoutoaccuratelyquantifytranscriptionalactivationinresponsetoSRFpathwayactivation.
