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Signosis/ELK-TAD Luciferase Reporter Hela Stable Cell Line/SL-0041-FP/1 Ea
Description:
ELKResponsiveLuciferaseReporterHelaCellLineisderivedhumancervicalcancer,andstablyexpressfireflyluciferasereportergeneunderthecontrolofELKresponseelement.ThiscelllineisanidealcellularmodelformonitoringtheactivationofMAPKresponsePathwaytriggeredbystimulitreatment,enforcedgeneexpressionandgeneknockdown.Principle:
Mitogen-activatedproteinkinase(MAPK)signalingpathwaysmediateimportanteukaryotictranscriptionalresponsestoextracellularsignalsthatbeginatthecellsurface. WhiledifferentexternalstimulimayactivatedistinctMAPKsignalingpathways,manyMAPKpathwayslaterconvergeintheactivationofETSdomaintranscriptionfactorELK1. ELK1isamajornuclearsubstrateforMAPKs,JNK,p38,andERKpathways,wherephosphorylationofELK1bythesekinasesresultsinaconformationalchangeofELK1andtriggersitsDNAbindingactivity.
SignosishasestablishedanELK-TADluciferasereporterstablecellline. ThiscelllineexpressesafusionproteinlinkingtheDNAtransactivationdomain(TAD)ofELKtoGal4. AstheTADisphosphorylated,thisGal4fusionproteinwilldriveluciferaseexpressioncontrolledbyGal4-UAS. ThiscelllinecanactasareportersystemtomonitorELK1-dependenttranscriptionalactivityandMAPKsignaling,astriggeredbystimulitreatment,enforcedgeneexpressionandgeneknockdown.
Data:
AnalysisofELK-TADReporterCellLineinresponsetoMEKKoverexpression.ThecellsweretransfectedwithvaryingconcentrationofMEKK. 48hoursaftertransfection,celllysatewascollectedtomeasuretheluciferaseactivity. ELK-TADreporteractivityshowedagenedose-dependentincreasetoMEKKoverexpression.
