>
产品中心 >
Stable_cell_line >
Signosis/TCF/LEF Luciferase Reporter CHO-K1 Stable Cell Line/SL-0028-NP/1 Ea
Description:
TCF/LEFResponsiveLuciferaseReporterCHO-K1StableCellLineisderivedfromChineseHamsterOvary,andstablyexpressfireflyluciferasereportergeneunderthecontrolofTCF/LEFresponseelement. Thiscelllineisanidealcellularmodelformonitoringtheactivation ofWnt/b-cateninSignalingPathwaytriggeredbystimulitreatment,enforcedgeneexpressionandgeneknockdown.Principle:
TheWntsignalingpathwayisimportanttobothembryonicdevelopmentandtumOrigenesis.β-Catenin,thecentralcomponentofthepathway,functionsasaco-factoroftheTCF/LEFfamilyoftranscriptionfactors. Together,theyactivatetranscriptionofWnttargetgenesbybindingtothepromotersofdownstreamtargetgenesinvolvedincellproliferation,survival,andmigration.
SignosishasestablishedaTCF/LEFluciferasereporterstablecelllinethathasbeenstablytransfectedwithpTA-TCF/LEF-luciferasereportervector,whichcontains6repeatsofTCF/LEFbindingsites,aminimalpromoterupstreamofthefireflyluciferasecodingregion. Therefore,thecelllinecanbeusedasareportersystemformonitoringtheactivationofβ-catenintriggeredbystimulitreatment,enforcedgeneexpressionandgeneknockdown.
PrinciplebehindTFluciferasereporter. TFluciferasereporterstablecelllineutilizesartificialpromoterconstructstodriveluciferaseexpression. Thepromoterregioncanconsistsofmultiplerepeatsofacis-elementTFbindingsite,aDNAfragmentfromthepromoterregionofaknownTFdownstreamgene,oraDNAfragmentcontainingputative/knownTFbindingsites. ThereareseveralwaysthataTFcanbeactivated,suchasthroughextracellularstimuliorthroughintracellularsignalingpathways. Onceactivated,theTFtranslocatestothenucleusandofteninteractswithrelevantco-factorstodrivegeneexpression. Onceluciferaseisexpressed,itcangeneratelightinanenzymaticassayandtheamountoflightmeasuredispositivelycorrelatedwiththelevelofTFactivation. |
Data:
AnalysisofSL-0028TCF/LEFLuciferaseReporterCHO-K1StableCellLine.CHO-k1cellsstablyexpressingTCF/LEFluciferasereporterweretreatedwith10mMLiClor40ng/mLWnt3atoactivateb-cateninsignaling. Thesecellsexhibitedtreatment-dependentincreaseinTCF/LEFluciferasereporteractivityafter16hourstreatmentperiod.
