>
产品中心 >
Stable_cell_line >
Signosis/TCF/LEF Luciferase Reporter Hela Stable Cell Line/SL-0022-NP/1 Ea
Description:
TCF/LEFResponsiveLuciferaseReporterHelaStableCellLineisderivedfromhumancervicalcancer,andstablyexpressfireflyluciferasereportergeneunderthecontrolofTCF/LEFresponseelement.ThiscelllineisanidealcellularmodelformonitoringtheactivationofWnt/b-cateninSignalingReceptorSignalingPathwaytriggeredbystimulitreatment,enforcedgeneexpressionandgeneknockdown.Principle:
TheWntsignalingpathwayisimportanttobothembryonicdevelopmentandtumOrigenesis.β-Catenin,thecentralcomponentofthepathway,functionsasaco-factoroftheTCF/LEFfamilyoftranscriptionfactors. Together,theyactivatetranscriptionofWnttargetgenesbybindingtothepromotersofdownstreamtargetgenesinvolvedincellproliferation,survival,andmigration.
SignosishasestablishedaTCF/LEFluciferasereporterstablecelllinethathasbeenstablytransfectedwithpTA-TCF/LEF-luciferasereportervector,whichcontains6repeatsofTCF/LEFbindingsites,aminimalpromoterupstreamofthefireflyluciferasecodingregion.Therefore,thecelllinecanbeusedasareportersystemformonitoringtheactivationofβ-catenintriggeredbystimulitreatment,enforcedgeneexpressionandgeneknockdown.
PrinciplebehindTFluciferasereporter. TFluciferasereporterstablecelllineutilizesartificialpromoterconstructstodriveluciferaseexpression. Thepromoterregioncanconsistsofmultiplerepeatsofacis-elementTFbindingsite,aDNAfragmentfromthepromoterregionofaknownTFdownstreamgene,oraDNAfragmentcontainingputative/knownTFbindingsites. ThereareseveralwaysthataTFcanbeactivated,suchasthroughextracellularstimuliorthroughintracellularsignalingpathways. Onceactivated,theTFtranslocatestothenucleusandofteninteractswithrelevantco-factorstodrivegeneexpression. Onceluciferaseisexpressed,itcangeneratelightinanenzymaticassayandtheamountoflightmeasuredispositivelycorrelatedwiththelevelofTFactivation. |
Data:
AnalysisofSL-0022TCF/LEFLuciferaseReporterHelaStableCellLine.HelacellsstablyexpressingTCF/LEFluciferasereporterweretreatedwith10mMLiClor40ng/mLWnt3atoactivateb-cateninsignaling. Thesecellsexhibitedtreatment-dependentincreaseinTCF/LEFluciferasereporteractivityafter24hourstreatmentperiod.
