>
产品中心 >
Stable_cell_line >
Signosis/BMP Responsive Luciferase Reporter HEK293 Cell Line/SL-0051-FP/1 Ea
Description:
SMAD1/5/8ResponsiveLuciferaseReporterHEK293CellLineisderivedhumanembryonickidney,andstablyexpressfireflyluciferasereportergeneunderthecontrolofSMAD1/5/8 responseelement.ThiscelllineisanidealcellularmodelformonitoringtheactivationofBMPresponsePathwaytriggeredbystimulitreatment,enforcedgeneexpressionandgeneknockdown.Principle:
Bonemorphogenicprotein(BMP)isinvolvedinembryogenesis,developmentofmanyorgansystemsandadulttissuehomeostasis.Smads(Smad1/5/8)areactivatedduringthesignaltransductionandthenformacomplexwithSmad4,translocateintothenucleuswheretheyregulateexpressionoftranscriptionalfactorsandtranscriptionalcoactivators. DeficiencyinBMPproductionorfunctionalityusuallyleadstomarkeddefectsorseverehumandiseasesassociatedwithmostorgansystems. MonitoringtheBMPactivityisessentialtoresearchoftheBMPsignaling-associateddiseasesandconductdrugdiscovery.
SignosishasestablishedaBMPluciferasereporterstablecelllinethathasbeenstablytransfectedwithpTA-BMP-luciferasereportervector,whichcontains4repeatsofBMPbindingsites,aminimalpromoterupstreamofthefireflyluciferasecodingregion.Therefore,thecelllinecanbeusedasareportersystemformonitoringtheactivationofBMPtriggeredbystimulitreatment,enforcedgeneexpressionandgeneknockdown.
PrinciplebehindTFluciferasereporter. TFluciferasereporterstablecelllineutilizesartificialpromoterconstructstodriveluciferaseexpression. Thepromoterregioncanconsistsofmultiplerepeatsofacis-elementTFbindingsite,aDNAfragmentfromthepromoterregionofaknownTFdownstreamgene,oraDNAfragmentcontainingputative/knownTFbindingsites. ThereareseveralwaysthataTFcanbeactivated,suchasthroughextracellularstimuliorthroughintracellularsignalingpathways. Onceactivated,theTFtranslocatestothenucleusandofteninteractswithrelevantco-factorstodrivegeneexpression. Onceluciferaseisexpressed,itcangeneratelightinanenzymaticassayandtheamountoflightmeasuredispositivelycorrelatedwiththelevelofTFactivation. |
Data:
AnalysisofSL-0051BMPreporteractivityinresponsetoBMPtreatment. Thecellswereseededona96-wellplateovernightincompletegrowthmedia. ThecellsthenweretreatedwithorwithoutBMPingrowthmediawith0.1%FBSfor16hours. TheBMPLuciferaseReporterHEK293CellLineexhibitsBMPdependentincreaseinluciferaseactivitywhencomparedtountreatedcells.
