Description:
Anti-dsDNAantibodiesthatappeartobecriticalinthepathogenesisoftissueinjuryarecharacteristicofsystemiclupuserythematosus(SLE).Thereisagoodcorrelationbetweenanti-dsDNAantibodylevelsanddiseaseactivity.Theoveralldetectionrateoftheseantibodiesisapproximately50-55%inSLEpatientsandabout89%inSLEpatientswithactiverenaldisease.Whentheyarepresentinhighconcentration,anti-dsDNAantibodiesarevirtuallyspecificforSLE(>90%).AntibodiestodsDNAmaydisappearwithimmunosuppressivetreatmentandduringremission.Theyrarelyoccurinotherautoimmunedisorders.Signosishasdevelopedanti-dsDNAELISA,asandwichquantitativeassay,toscreenthepresenceofserumds-DNAantibodiesIgG.Principle:
AutoimmuneELISAkitsmeasureautoimmuneantibodiesintheserum.Itisbasedontheprincipleofasolidphaseenzyme-linkedimmunosorbentassay.Theassayutilizesaspecificantigenforimmobilizationonthemicrotiterwellsandanti-humanIgGantibodyconjugatedtohorserADIshperoxidase(HRP)fordetection.Thetestsampleisallowedtoreactsimultaneouslywiththetwocomponents,resultingin autoimmuneantibodiesbeingsandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Afterincubation,thewellsarewashedtoremoveunbound-labeledantibodies.AHRPsubstrate,TMB,isaddedtoresultinthedevelopmentofabluecolor.ThecolordevelopmentisthenstoppedwiththeadditionofStopSolutionchangingthecolortoyellow.Theconcentrationofautoimmuneantibodies isdirectlyproportionaltothecolorintensityofthetestsample.Absorbanceismeasuredspectrophotometricallyat450nm.
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